Fluorescein Chemicals are functional is functional as a fluorescent tracer suited for several applications. These are suited for microscopy and are suited as the advanced dye laser. The chemicals are used for forensics and serology as these assist in detecting dye tracing as well as latent blood stains. Fluorescein Chemicals have a significant role in radioopaque medium. The chemicals are functional as the gamma-lactone and are useful as the organic heteropentacyclic compound.